- Specially-appointed Professor (Part-time)
- Department of Applied Biological Sciences, Faculty of Bioenvironmental Sciences
Research Field | Molecular and cellular biology, Applied microbiology, Ecological molecular microbial physiology |
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Affiliated Academic Societies | Japan Society for Bioscience, Biotechnology, and Agrochemistry, The Japanese Biochemical Society, Yeast Genetics Society of Japan, The Molecular Biology Society of Japan |
Academic Degrees | PhD. Degree in Agriculture, Kyoto University |
Brief Biography | University Education Scientific Positions Adjunct Associate Professor, National Institute for Basic Biology（2000-2005） |
Achievements and Awards | -Fellowship: Kumura Fellowship (1982-1988), Overseas Study Fellowship from the Uehara Memorial Foundation (1996) |
Related Links |
Research Overview
- C1- metabolism, and their regulation (identification and discoveries of HPS-PHI carbon fixing genes in versatile microbes, development of yeast gene expression system、symbiotic system between plants and C1-microbes, microbe-plant interaction .)
- Molecular mechanism and physiology of intracellular material cycle (molecular mechanism for methanol-inducible gene expression and organellar dynamics, molecular mechanism of microautophgay, physiological role of organellar turnover during plant pathogenesis in microbes).
- Development of Redoxfluor, an intracellular redox sensor and redox metabolism
Research Keywords | Methanol, methane, material circulation, autophagy, organelle dynamics, peroxisome, vacuole, lipid droplet, microbe-plant interactions |
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Academic Papers | Yasuyoshi Sakai, Tomohiro Rogi, Tetsu Yonehara, Nobuo Kato, and Yoshiki Tani. High-level ATP production by a genetically-engineered Candida yeast. Nature Biotechnol., 12, 291-293 (1994). Yasuyoshi Sakai, Antonius Koller, Linda K. Rangell, Gilbert A. Keller, and Suresh Subramani. Peroxisome degradation by microautophagy in Pichia pastoris: Identification of specific steps and morphological intermediates. J. Cell Biol., 141, 625-636 (1998). Masahide Oku, Dirk Warnecke, Takeshi Noda, Frank Müller, Ernst Heinz, Hiroyuki Mukaiyama, Nobuo Kato, and Yasuyoshi Sakai. Peroxisome degradation requires catalytically active sterol glucosyltransferase with a GRAM domain. EMBO J., 22, 3231-3241 (2003). Masahide Oku, Yuichiro Maeda, Yoko Kagohashi, Takeshi Kondo, Mai Yamada, Toyoshi Fujimoto, and Yasuyoshi Sakai. Evidence for ESCRT- and clathrin-dependent microautophagy. J. Cell Biol., 216, 3263-3274 (2017). Makoto Asakura, Sachiko Ninomiya, Miki Sugimoto, Masahide Oku, Shun-ichi Yamashita, Tetsuro Okuno, Yasuyoshi Sakai, and Yoshitaka Takano. Atg26-mediated pexophagy is required for host invasion by the plant pathogenic fungus Colletotrichum orbiculare. Plant Cell, 21, 1291-1304 (2009). [In Brief] [京都新聞、日本工業新聞に紹介記事] Kosuke Kawaguchi, Hiroya Yurimoto, Masahide Oku, and Yasuyoshi Sakai. Yeast methylotrophy and autophagy in a methanol-oscillating environment on growing Arabidopsis thaliana leaves. PLoS One, 6, e25257 (2011). . [京都新聞に紹介記事] Hiroyuki Iguchi, Hiroya Yurimoto, and Yasuyoshi Sakai. Stimulation of methanotrophic growth in co-cultures by cobalamin excreted by rhizobia. Appl. Environ. Microbiol., 77, 8509-8515 (2011). Naoko Yoshida, Hiroyuki Iguchi, Hiroya Yurimoto, Akio Murakami, and Yasuyoshi Sakai. Aquatic plant surface as a niche for methanotrophs. Front. Microbiol., 5, 30 (2014) Hiroya Yurimoto, Hiroyuki Iguchi, Do Thi Di Thien, Akio Tani, Yutaka Okumoto, Atushi Ota, Takahiro Yamauchi, Takahiro Akashi, and Yasuyoshi Sakai. Methanol bioeconomy: promotion of rice crop yield in paddy fields with microbial cells prepared from natural gas-derived C1 compound. Microb. Biotechnol., 14, 1385-1396 (2021). Kosuke Shiraishi and Yasuyoshi Sakai. Autophagy as a survival strategy for eukaryotic microbes living in the phyllosphere. Front. Plant Sci., 13, 867486 (2022). Shin Ohsawa, Masahide Oku, Hiroya Yurimoto, and Yasuyoshi Sakai. Regulation of peroxisome homeostasis by post-translational modification in the methylotrophic yeast Komagataella phaffii. Front. Cell Dev. Biol., 10, 887806 (2022). Yasuyoshi Sakai and Masahide Oku. ATG and ESCRT control multiple modes of microautophagy. FEBS Lett., 598, 48-58 (2024). |
Publications
Masahide Oku and Yasuyoshi Sakai. Pexophagy in Pichia pastoris. In “Methods in Enzymology, Autophagy: Lower Eukaryotes and Non-Mammalian Systems, Part A”, Dan Klionsky ed., Elsevier Inc., 451, 217-228 (2008))
Shun-ichi Yamashita, Masahide Oku, Yasuyoshi Sakai, Yukio Fujiki, Experimental Systems to Study Yeast Pexophagy. Methods Mol. Biol., 595, 249-255 (2017).
Hiroyuki Iguchi, Hiroya Yurimoto, and Yasuyoshi Sakai. Methylovulum Iguchi, Yurimoto and Sakai 2011, 813VP. Bergey’s Manual of Systematics of Archaea and Bacteria,1-4 (2016).